AcP/AcK-based ATP Regeneration System

Use Case

AcP/AcK-based ATP Regeneration System

Biotechnology, Pharma, Biochemistry, Biocatalysis, Green Chemistry

Many enzymatic reactions require ATP as a co-substrate, and the availability of ATP can be a limiting factor in biocatalytic processes. To overcome this challenge, ATP regeneration systems have been developed, enabling sustainable and cost-effective biocatalytic synthesis. ATP regeneration systems involve coupling the desired biocatalytic reaction with an ATP-generating enzyme. These ATP-generating enzymes are carefully selected and optimized to work in tandem with the target biocatalyst, allowing for efficient and continuous ATP recycling during the biocatalytic process. Advantages of ATP regeneration: Ensuring constant adequate ATP levels throughout the process generally improves yield and reaction velocity of an enzymatic reaction. ATP regeneration may be used to reduce starting ATP levels to overcome inhibitory effects in single or coupled reactions. By utilizing renewable and inexpensive substrates for ATP generation, ATP regeneration systems can reduce the reliance on costly and resource-intensive sources of ATP. There are different possibilities for enzymatic ATP regeneration: Acetate kinase using acetyl phosphate as phosphate donor Creatine kinase using creatine phosphate as phosphate donor Pyruvate kinase using phosphoenolpyruvate as phosphate donor Polyphosphate kinase using polyphosphate as phosphate donor