Use Case

Biopsy Tissue from Dog with Oral Melanoma for Cancer Diagnostics/Biomarker Development and Cell Line Generation

Use Case

Biopsy Tissue from Dog with Oral Melanoma for Cancer Diagnostics/Biomarker Development and Cell Line Generation

Description

This study aimed to establish two- and three-dimensional primary cell lines from oral canine melanomas using fine-needle aspiration, a minimally invasive sampling method. Samples were collected from six dogs, including four primary oral melanomas and five lymph node metastases. The cells were processed into single-cell suspensions and seeded in flasks or formed into organoids using Matrigel. Characterization was performed using flow cytometry with antibodies against Melan-A, PNL2, and Sox-10. This approach provides a non-invasive means to obtain cell samples, particularly beneficial for patients unsuitable for surgery, and enables the creation of in vitro models for comparative studies in mucosal melanoma oncology. Notably, this is the first study to establish neoplastic primary cell cultures from dogs using fine-needle aspiration. [Ref: Lo Giudice, A., Porcellato, I., Pellegrini, M., Rottenberg, S., He, C., Dentini, A., Moretti, G., Cagiola, M., Mechelli, L., Chiaradia, E., & Brachelente, C. (2024). Establishment of Primary Cell Cultures from Canine Oral Melanomas via Fine-Needle Aspiration: A Novel Tool for Tumorigenesis and Cancer Progression Studies. Animals, 14(13), 1948. https://doi.org/10.3390/ani14131948]

Details

Industries:

Cancer Research, Biomarker Development, Cell Line Generation

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Biopsy Tissue from Dog with Oral Melanoma for Cancer Diagnostics/Biomarker Development and Cell Line Generation is a Use Case of:

Service

Canine Biopsy - Oral Melanoma

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