Cytek Biosciences

cFluor® V505 Anti-Human HLA-DR (L243) | Cytek Biosciences

The L243 monoclonal antibody binds to the human HLA-DR antigen, a 36-kDa alpha heavy chain and a 27-kDa beta light chain heterodimeric cell surface glycoprotein in transmembrane major histocompatibility complex 2 (MHC II) family (1)(2). HLA-DR, also known as human leukocyte antigen DR isotype, is present on the surface of antigen-presenting cells, including B cells, dendritic cells, macrophages, monocytes and activate T cells. MHC class II regulates the immune system by playing a critical role in binding and presenting antigen-derived peptides to peptide-MHC II-specific CD4 T cells(3). The antibody was conjugated to a fluorophore and purified by affinity chromatography. PRODUCT DETAILS Clone:                  L243 Reactivity:          Human, African Green, Baboon, Chimpanzee, Cynomolgus, Dog (Canine), Marmoset, Rhesus, Squirrel Monkey, Tamarin Format:               cFluor® V505  Isotype:               Mouse IgG2a, κ Tested Dilution:  5 μL / test Application:        Flow Cytometry Storage:               2-8°C and protected from Light. Do not freeze Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA). Data Sheets:       TDS SDS   RECOMMENDED USAGE Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product.        Spectral signature of cFluor® V505 from a Cytek® Aurora 5 laser system equipped with 355 nm, 405 nm, 488 nm, 561 nm and 640 nm lasers using CytekAssaySettings.  REFERENCES Korman, A J et al. 1982. Proc Natl Acad Sci U S A. 79(19):6013-6017 Shackelford DA, et al. 1982. Immunol Rev. 66:133-87 Walseng E, et al. 2008. J Biol Chem. 23;283(21):14717-27  

redFluor™ 710 Anti-Human HLA-DR (L243) | Cytek Biosciences

TDS | SDS The L243 antibody reacts with a member of the human MHC Class II antigens, HLA-DR. The HLA-DR antigen is expressed on B lymphocytes, activated T lymphocytes, activated NK cells, monocytes, macrophages, other antigen presenting cells and progenitor cells. The L243 antibody is specific to an epitope on the alpha subunit of the heterodimeric HLA-DR protein and binds a different epitope than the LN3 antibody clone. It does not cross-react with HLA-DP or HLA-DQ. This antibody is reported to be cross-reactive with non-human primates including Chimpanzee, Cynomolgus, Rhesus and Baboon. Please choose the appropriate format for each application. Name redFluor™ 710 Anti-Human HLA-DR (L243) Cat. No. 80-9952 Gene ID 3122 Clone L243 Isotype Mouse IgG2a, κ Reactivity Human Cross Reactivity Chimpanzee, Cynomolgus, Rhesus, Baboon, Squirrel Monkey, African Green Monkey Format redFluor™ 710 Application Flow Cytometry