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Scripps Laboratories
San Diego, United States
B
11-50 Employees
1984
Key takeaway
Scripps Laboratories specializes in protein purification, developing techniques that preserve protein integrity and enhance performance. Their expertise extends to a range of products, including native proteins, antibodies, and recombinant proteins.
Reference
Core business
Protein Purification Experts | ScrippsLabs.com
FLUOROGENICS LIMITED
Salisbury, United Kingdom
A
1-10 Employees
2011
Key takeaway
NEB offers a range of products relevant to protein purification, including the Authenticase mix of structure-specific nucleases that targets mismatches and indel regions on double-stranded DNA. Their NEBNext UltraExpress™ kits also facilitate efficient library preparation, which can be beneficial for downstream applications in protein analysis.
Reference
Product
Protein Purification | NEB
Peak Proteins
Macclesfield, United Kingdom
A
11-50 Employees
-
Key takeaway
Peak Proteins, a trading name of Sygnature Discovery Limited, offers specialized services in protein expression and purification, focusing on designing and producing customized soluble and membrane proteins to support various stages of the drug discovery process.
Reference
Service
Protein Expression & Purification | Peak Proteins
Protein Expression and Purification services to design, express and purify customised soluble and membrane proteins reagents to support all stages of the drug discovery process.
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e-Proteins
Gembloux, Belgium
A
1-10 Employees
2012
Key takeaway
e-Proteins specializes in the production of avidin derivatives, which are key in protein purification applications, offering reliable and standardized products that enhance performance. Their commitment to quality and innovative R&D ensures consistent and effective solutions for biotin binding applications.
Reference
Core business
e-Proteins: A Solution of any biotin Binding application
e-Proteins is the world leader in avidin derivatives/binding protein production which are the best alternatives to Streptavidin.
ProteinArk Ltd
Sheffield, United Kingdom
A
11-50 Employees
-
Key takeaway
The company, Protein Ark, offers a range of products that may be relevant for protein purification, including unique spin columns for batch control incubation with chromatography resins and various FPLC columns. Their innovative solutions like the Super Co-NTA Affinity HP Resin and quick Coomassie stain can enhance protein purification processes.
Reference
Core business
Protein Ark – Custom Services – Protein Ark – The Protein Company
Aalto Scientific LTD.
Eatonton, United States
B
51-100 Employees
1979
Key takeaway
Aalto Scientific specializes in the manufacture of purified human proteins, offering a complete line suitable for control sera manufacturing or antibody development. With over 40 years of experience and a state-of-the-art facility, Aalto is well-equipped to meet custom specifications in protein purification.
Reference
Service
Protein Purification | Contract Manufacturing | Aalto Scientific
Profacgen
Shirley, United States
B
- Employees
-
Key takeaway
Profacgen is a leading protein service provider that offers custom protein purification services, utilizing a variety of expression systems and developing tailored protocols to meet customer specifications. Their expertise also extends to protein interaction analysis, providing a comprehensive range of assays for validation and kinetic studies.
Reference
Core business
Profacgen, Perfect Protein
Creative Proteomics
Shirley, United States
B
51-100 Employees
2005
Key takeaway
Creative Proteomics offers specialized protein purity and homogeneity characterization services, essential for quality control before further processing of proteins. Their advanced proteome analysis platform ensures high-throughput and sensitive protein separation, characterization, identification, and quantification.
Reference
Service
Protein Purity and Homogeneity Characterization Service - Creative Proteomics
Creative Proteomics provides protein purity and homogeneity characterization services for quality control of prior to using protein for further processes.
MyBio Ltd
Ireland
A
1-10 Employees
2009
Key takeaway
MyBio offers the MagneHis Protein Purification System, which utilizes a magnetic-based method for the rapid purification of polyhistidine- or HQ-tagged proteins. This system is part of their extensive range of high-quality bioscience products, including antibodies and cytokines from Proteintech.
Reference
Product
MagneHis Protein Purification System – MyBio Ireland
The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.
Alpha Biotech Ltd.
Glasgow, United Kingdom
A
1-10 Employees
2002
Key takeaway
AlphaBiotech offers the Protein Maker workstation, which simplifies high throughput protein purification chromatography by allowing parallel processing of 24 columns and 96 samples unattended. This innovative system accelerates research on protein function, making it a valuable tool for laboratories engaged in protein purification.
Reference
Product
Protein Maker - AlphaBiotech Limited
Accelerate your research on protein function with parallel purification. The Protein Maker workstation makes high throughput protein purification chromatography simple by allowing you to run 24 columns (1-5 ml) in parallel, and process 96 samples unattended. Protein Maker’s flexible design allows you to customize sample and column types to achieve a variety of tasks: … Protein Maker Read More »
Technologies which have been searched by others and may be interesting for you:
Protein purification is a series of processes designed to isolate a specific protein from a complex mixture, such as a cell lysate or tissue extract. This technique is crucial in biochemistry and molecular biology for studying protein structure, function, and interactions. The process often involves multiple steps, including cell lysis, centrifugation, and various chromatographic techniques. Each method targets specific properties of proteins, such as size, charge, or affinity for certain ligands. Successful purification results in a high degree of purity, allowing for more accurate analysis and application in research, diagnostics, and therapeutic development.
Protein purification involves several techniques aimed at isolating a specific protein from a complex mixture, such as cell lysates or tissue extracts. Initially, the process may start with the extraction of proteins, typically using a buffer solution that helps to dissolve the proteins while maintaining their stability. Following extraction, various methods are employed to separate the target protein from others based on different properties. Common techniques include affinity chromatography, which utilizes specific interactions between the protein and a ligand, and gel electrophoresis, where proteins are separated based on size and charge. Additionally, ultrafiltration and precipitation methods may be applied to further refine the purification process. Each method contributes to achieving high purity levels necessary for subsequent analytical or functional studies.
1. Affinity Chromatography
This method exploits specific interactions between a target protein and a ligand attached to a solid support. The protein of interest binds to the ligand, allowing for effective separation from other proteins.
2. Ion Exchange Chromatography
Ion exchange chromatography separates proteins based on their charge. Proteins are loaded onto a column containing charged resin, where they interact based on their net charge, resulting in distinct elution profiles.
3. Gel Filtration Chromatography
This technique separates proteins based on their size. Larger proteins pass through the column more quickly, while smaller proteins take longer as they enter the pores of the gel, allowing for effective size-based separation.
4. Precipitation
Precipitation involves adding a precipitating agent to a protein solution, causing the target protein to aggregate and separate from the solution. This method is often used as a preliminary step before more refined purification techniques.
5. Ultrafiltration
Ultrafiltration employs membranes with specific pore sizes to separate proteins based on molecular weight. This technique is useful for concentrating proteins and removing small contaminants.
6. High-Performance Liquid Chromatography (HPLC)
HPLC is a precise method that uses high pressure to pass a liquid sample through a column filled with packing material. It allows for the efficient separation and purification of proteins based on their interactions with the column material.
Protein purification is essential in research as it allows scientists to isolate specific proteins from complex mixtures. This isolation is crucial for understanding protein structure and function, as well as for studying interactions with other biomolecules. By purifying proteins, researchers can conduct detailed analyses, such as determining enzymatic activity and characterizing binding properties. Furthermore, purified proteins are necessary for various applications, including drug development, vaccine production, and diagnostic assays. Without effective protein purification methods, the reliability of experimental results would diminish, hindering advancements in biotechnology and medicine.
To effectively carry out protein purification, several types of equipment are essential.
1. Centrifuge
This device is crucial for separating proteins from cellular debris based on density.
2. Chromatography Systems
These systems, including affinity, ion exchange, and size exclusion chromatography, are utilized for isolating proteins based on various properties.
3. Spectrophotometer
A spectrophotometer is important for measuring protein concentration and assessing purity through absorbance at specific wavelengths.
4. Gel Electrophoresis Apparatus
This equipment is used for analyzing protein size and purity by separating proteins in a gel matrix.
5. Dialysis Equipment
Dialysis is necessary for removing small molecules and salts from protein solutions to enhance purity.
6. Refrigerated Storage Units
Maintaining proteins at the correct temperature is vital for preserving their stability and functionality during purification processes.
Utilizing these pieces of equipment ensures an efficient and effective protein purification process, leading to high-quality protein samples for further study or application.
Some interesting numbers and facts about your company results for Protein Purification
| Country with most fitting companies | United States |
| Amount of fitting manufacturers | 5010 |
| Amount of suitable service providers | 3745 |
| Average amount of employees | 11-50 |
| Oldest suiting company | 1979 |
| Youngest suiting company | 2012 |
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Some interesting questions that has been asked about the results you have just received for Protein Purification
What are related technologies to Protein Purification?
Based on our calculations related technologies to Protein Purification are Biomedical (Red), Bioinformatics (Gold), Environmental Biotechnology (Grey), Agricultural Biotechnology (Green), Food Related Biotechnology (Yellow)
Which industries are mostly working on Protein Purification?
The most represented industries which are working in Protein Purification are Biotechnology, Other, Pharmaceuticals, Chemicals, Healthcare
How does ensun find these Protein Purification Companies?
ensun uses an advanced search and ranking system capable of sifting through millions of companies and hundreds of millions of products and services to identify suitable matches. This is achieved by leveraging cutting-edge technologies, including Artificial Intelligence.
